Searchable abstracts of presentations at key conferences in endocrinology

ea0020p628 | Reproduction | ECE2009

Obesity resulting from Gs-alpha mutations in the maternal (but not paternal) allele is a consequence of Gs-alpha imprinting in the central nervous system

Esponda Pedro , Relloso Miguel

The female genital tract of the mouse was in vivo transfected using the reporter gene β-galactosidase and the liposome Lipofectamine as gene vector. All animals used were anaesthetised. DNA/Liposome complexes were injected through the infundibulum of the tubes in adult, immature and pseudopregnant females. Females that were at different stages of the ovarian cycle were also used. Transfection was analyzed using histochemical, immunological and molecular (Southern b...